Imaging of adpotive cell transfer

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Non-invasive imaging of endothelial cell (EC) surface markers holds promise in identifying early signs of inflammation, angiogenesis or atherosclerosis in biological systems.

We previously devised a reporter of E-selectin expression based on nano-sized iron oxide particles linked to a highly specific, high affinity anti-human E-selectin antibody fragment.

The experiments with targeted imaging agent suggested that the conjugates were specifically bound to EC only if marker expression was specifically induced. Specific binding of particles to EC resulted in a strong MR T2-weighted signal change. This suggested that targeted MR imaging detects inducible expression of cell-adhesion molecules.

We are currently investigating:

1. xenogeneic blood neovessels

2. targeted MR probes with the specificity against human endothelial surface markers

3. monitoring of vascularization in engineered artificial tissue grafts

Collaborators:

Dr. Chris Sotak, Worcester Polytechnic Institute

Dr. Bill Luscinskas, Brigham and Womens Hospital

Recent Publications

Kang HW, Walvick R, Bogdanov A Jr. In vitro and In vivo imaging of antivasculogenesis induced by Noggin protein expression in human venous endothelial cells. FASEB J. Aug 19 [Epub ahead of print].

Bogdanov AA Jr, Lin CP, Kang HW. Optical imaging of the adoptive transfer of human endothelial cells in mice using anrti-CD31 monoclonal antibody. Pharm Res 2007 24:1186-1192.

Kang HW, Torres D, Wald L, Weissleder R, Bogdanov A Jr. Targeted imaging of human endothelial-specific marker in a model of adoptive cell transfer. Lab Invest 2006, 86:599-609.

Branching of human endothelial cells formed in vivo in Matrigel^TM and mouse supporting cells. CD31 staining (red fluorescence), nuclei stained with DAPI (blue).

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